Journal: Applied physics reviews

Article Title: Modulating physical, chemical, and biological properties in 3D printing for tissue engineering applications

doi: 10.1063/1.5050245

Figure Lengend Snippet: (A) Schematic of 3D bioprinting of GelMA-coated gold nanorod nanocomposite bioinks to form a cardiac tissue construct. (Reproduced with permission from K. Zhu et al., Adv. Funct. Mater. 27, 1605352 (2017). Copyright 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim). (B) 3D printed microfish made from PEGDA with embedded iron oxide and platinum nanoparticles to enable propulsion and magnetic control. (Reproduced with permission from W. Zhu et al., Adv. Mater. 27, 4411 (2015). Copyright 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim). (C) Bioinspired liver detoxification device comprised of PEGDA embedded with polydiacetylene (PDA) nanoparticles for toxin neutralization. (Reproduced with permission from M. Gou et al., Nat. Commun. 5, 3774 (2014). Copyright 2014 Zhu et al.). (D1) Printed concentric circle structure with gradients of hydroxyapatite nanoparticles and complementary image stained with Alizarin Red. (D2) A printed bone-like structure with varied gradient concentrations of hydroxyapatite nanoparticles stained with Alizarin Red. (D3) Corresponding quantified staining intensities of Alizarin Red in the concentric circle and bone-like structures. (D4) Seeded preosteoblasts on gradients of hydroxyapatite hydrogel slabs. (Reproduced with permission from W. Liu et al., Adv. Mater. 29, 1604630 (2017). Copyright 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim). (E) Overview of 3D printed biohybrid osteochondral gradient hydrogel scaffolds. (Reproduced with permission from F. Gao et al., Adv. Funct. Mater. 28, 1706644 (2018). Copyright 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim).

Article Snippet: Copyright 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim). (E) Overview of 3D printed biohybrid osteochondral gradient hydrogel scaffolds. (Reproduced with permission from F. Gao et al. , Adv.

Techniques: Construct, Control, Neutralization, Staining

Journal: Applied physics reviews

Article Title: Modulating physical, chemical, and biological properties in 3D printing for tissue engineering applications

doi: 10.1063/1.5050245

Figure Lengend Snippet: Summary of approaches for 3D printed functional tissues. a

Article Snippet: Copyright 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim). (E) Overview of 3D printed biohybrid osteochondral gradient hydrogel scaffolds. (Reproduced with permission from F. Gao et al. , Adv.

Techniques: Functional Assay, Migration, Irradiation, Construct, Micro-CT, High Throughput Screening Assay, Encapsulation

Journal: Scientific Reports

Article Title: 3D bioprinting of fish skin-based gelatin methacryloyl (GelMA) bio-ink for use as a potential skin substitute

doi: 10.1038/s41598-024-73774-1

Figure Lengend Snippet: Adding conditions of methacrylic anhydride at various concentrations.

Article Snippet: The 3D GelMA hydrogel scaffold was designed with Cellink Heart Ware Repetier – Host Software version 2.1.3 and printed using a 3D bioprinter (CELLINK, Gothenburg, Sweden) in a honeycomb square shape with dimensions of 20 × 20 × 2 mm 3 .

Techniques:

Journal: Scientific Reports

Article Title: 3D bioprinting of fish skin-based gelatin methacryloyl (GelMA) bio-ink for use as a potential skin substitute

doi: 10.1038/s41598-024-73774-1

Figure Lengend Snippet: Critical-sized full-thickness skin defect procedure and 3D GelMA hydrogel scaffold transplantation. ( A-C ) Under anesthesia, the dorsal mid-lumbar region of the rat was shaved and cleaned, and the defect size was marked. ( D ) A critical-sized (2 × 2 cm 2 ) square-shape piece of skin was excised, and ( E-G ) the defect was transplanted with either GelMA alone or GelMA + ASCs + HPL. (H) The wound was covered with BACTIGRAS antiseptic dressing, gauze, and Tegaderm transparent film dressing. (I) To secure the transplant in place, four stitches attaching the bandage to the skin were applied.

Article Snippet: The 3D GelMA hydrogel scaffold was designed with Cellink Heart Ware Repetier – Host Software version 2.1.3 and printed using a 3D bioprinter (CELLINK, Gothenburg, Sweden) in a honeycomb square shape with dimensions of 20 × 20 × 2 mm 3 .

Techniques: Transplantation Assay

Journal: Scientific Reports

Article Title: 3D bioprinting of fish skin-based gelatin methacryloyl (GelMA) bio-ink for use as a potential skin substitute

doi: 10.1038/s41598-024-73774-1

Figure Lengend Snippet: 1 H-NMR spectra of obtained GelMA samples compared to fish skin gelatin.

Article Snippet: The 3D GelMA hydrogel scaffold was designed with Cellink Heart Ware Repetier – Host Software version 2.1.3 and printed using a 3D bioprinter (CELLINK, Gothenburg, Sweden) in a honeycomb square shape with dimensions of 20 × 20 × 2 mm 3 .

Techniques:

Journal: Scientific Reports

Article Title: 3D bioprinting of fish skin-based gelatin methacryloyl (GelMA) bio-ink for use as a potential skin substitute

doi: 10.1038/s41598-024-73774-1

Figure Lengend Snippet: Degree of substitution of fish skin GelMA samples.

Article Snippet: The 3D GelMA hydrogel scaffold was designed with Cellink Heart Ware Repetier – Host Software version 2.1.3 and printed using a 3D bioprinter (CELLINK, Gothenburg, Sweden) in a honeycomb square shape with dimensions of 20 × 20 × 2 mm 3 .

Techniques:

Journal: Scientific Reports

Article Title: 3D bioprinting of fish skin-based gelatin methacryloyl (GelMA) bio-ink for use as a potential skin substitute

doi: 10.1038/s41598-024-73774-1

Figure Lengend Snippet: DSC thermogram of the fish skin GelMA samples.

Article Snippet: The 3D GelMA hydrogel scaffold was designed with Cellink Heart Ware Repetier – Host Software version 2.1.3 and printed using a 3D bioprinter (CELLINK, Gothenburg, Sweden) in a honeycomb square shape with dimensions of 20 × 20 × 2 mm 3 .

Techniques:

Journal: Scientific Reports

Article Title: 3D bioprinting of fish skin-based gelatin methacryloyl (GelMA) bio-ink for use as a potential skin substitute

doi: 10.1038/s41598-024-73774-1

Figure Lengend Snippet: ( A ) Bar graph and ( B ) trend curve showing the swelling rate of the fish skin GelMA samples over a 24-h period. Data are expressed as mean ± SD ( n = 3/sample).

Article Snippet: The 3D GelMA hydrogel scaffold was designed with Cellink Heart Ware Repetier – Host Software version 2.1.3 and printed using a 3D bioprinter (CELLINK, Gothenburg, Sweden) in a honeycomb square shape with dimensions of 20 × 20 × 2 mm 3 .

Techniques:

Journal: Scientific Reports

Article Title: 3D bioprinting of fish skin-based gelatin methacryloyl (GelMA) bio-ink for use as a potential skin substitute

doi: 10.1038/s41598-024-73774-1

Figure Lengend Snippet: ( A ) A compressive strength test of the fish skin GelMA samples ( n = 3/sample). Data are expressed as mean ± SD. Statistically significant differences were assessed between the two experimental groups as indicated. ( B ) Printability of the fish skin GelMA95 sample at a concentration of 10% of PBS (w/v) after 8, 15, and 30 min of bio-ink preparation.

Article Snippet: The 3D GelMA hydrogel scaffold was designed with Cellink Heart Ware Repetier – Host Software version 2.1.3 and printed using a 3D bioprinter (CELLINK, Gothenburg, Sweden) in a honeycomb square shape with dimensions of 20 × 20 × 2 mm 3 .

Techniques: Concentration Assay

Journal: Scientific Reports

Article Title: 3D bioprinting of fish skin-based gelatin methacryloyl (GelMA) bio-ink for use as a potential skin substitute

doi: 10.1038/s41598-024-73774-1

Figure Lengend Snippet: ( A ) Viscosity and shear stress profiles relative to shear rate of the 10% w/v fish skin GelMA solution at 25 °C, ( B ) Temperature sweep test of the 10% w/v fish skin GelMA solution.

Article Snippet: The 3D GelMA hydrogel scaffold was designed with Cellink Heart Ware Repetier – Host Software version 2.1.3 and printed using a 3D bioprinter (CELLINK, Gothenburg, Sweden) in a honeycomb square shape with dimensions of 20 × 20 × 2 mm 3 .

Techniques: Viscosity, Shear

Journal: Scientific Reports

Article Title: 3D bioprinting of fish skin-based gelatin methacryloyl (GelMA) bio-ink for use as a potential skin substitute

doi: 10.1038/s41598-024-73774-1

Figure Lengend Snippet: The cell viability (%) of ASCs in the 3D GelMA hydrogel scaffold over 120 h. Data are expressed as mean ± SD. Statistical differences were assessed between the two experimental groups at the same time-point as indicated.

Article Snippet: The 3D GelMA hydrogel scaffold was designed with Cellink Heart Ware Repetier – Host Software version 2.1.3 and printed using a 3D bioprinter (CELLINK, Gothenburg, Sweden) in a honeycomb square shape with dimensions of 20 × 20 × 2 mm 3 .

Techniques:

Journal: Scientific Reports

Article Title: 3D bioprinting of fish skin-based gelatin methacryloyl (GelMA) bio-ink for use as a potential skin substitute

doi: 10.1038/s41598-024-73774-1

Figure Lengend Snippet: ( A ) Photographs of square wound closure kinetics at day 0, 3, 7, 10, and 14 during the wound healing process of three experimental groups: untreated, GelMA, and GelMA + ASCs + HPL. ( B ) The percentage of unclosed wound area for each experimental group; data are expressed as mean ± standard error of the mean ( n = 9/ group). ( C ) Representative SHG gray scale intensity images of collagen deposition from biopsy samples taken at day 14 post-wound creation. ( D ) Representative histological images of Masson’s trichrome (MT) staining of wound Sect. (40×): (i) untreated wound, wound treated with either (ii) GelMA or (iii) GelMA + ASCs + HPL at day 14. Blue staining indicates collagen fiber formation. GelMA + ASCs + HPL demonstrated deep blue staining compared with the other groups (untreated wound and wound treated with GelMA). Higher magnification (200 ×) of immunohistochemistry staining for type I collagen enlarged from the black dotted square of each correspondence image. Scale bars are 100 μm in ( C ) and 500 μm in ( D ).

Article Snippet: The 3D GelMA hydrogel scaffold was designed with Cellink Heart Ware Repetier – Host Software version 2.1.3 and printed using a 3D bioprinter (CELLINK, Gothenburg, Sweden) in a honeycomb square shape with dimensions of 20 × 20 × 2 mm 3 .

Techniques: Staining, Immunohistochemistry

Journal: Scientific Reports

Article Title: 3D bioprinting of fish skin-based gelatin methacryloyl (GelMA) bio-ink for use as a potential skin substitute

doi: 10.1038/s41598-024-73774-1

Figure Lengend Snippet: ( A ) Histological images of wound sections stained with anti-CD31 for the untreated wound and wounds treated with GelMA or GelMA + ASCs + HPL at day 14. New blood vessel formation (neovascularization) is indicated by arrowheads. Bar = 50 μm. ( B ) The number of blood vessels on day 14 of the wound treated with GelMA + ASCs + HPL was significantly higher than the untreated wound at the wound edge and wound bed.

Article Snippet: The 3D GelMA hydrogel scaffold was designed with Cellink Heart Ware Repetier – Host Software version 2.1.3 and printed using a 3D bioprinter (CELLINK, Gothenburg, Sweden) in a honeycomb square shape with dimensions of 20 × 20 × 2 mm 3 .

Techniques: Staining

Journal: International Journal of Molecular Sciences

Article Title: Sarcoma Spheroids and Organoids—Promising Tools in the Era of Personalized Medicine

doi: 10.3390/ijms19020615

Figure Lengend Snippet: Comparison of various tumor models.

Article Snippet: 3D scaffold-based cell cultures Cells seeded in structures of different materials: hydrogels, 3D life biomimetic, 3D InsertTM scaffolds (synthetic bone derivatives), Alvetex ® , - Cellular heterogeneity: Different cell types can be grown in the hydrogel; cellular 3D organization occurs spontaneously; necrotic zones may be formed - Gene expression: Gene expression and cell composition similar to in vivo tumors - Drug screening: Suitable to investigate drug penetration and interactions of cells with bone derivatives; spheroids obtainable in 2–7 days - Useful to study hypoxia , - Some expensive materials and special equipment required; - Extensive handling necessary, time-consuming; - Reproducibility is highly dependent on the technique used to produce scaffold , - The size of spheroids is not homogeneous and is generally around 50 μm - The ECM formed is artificial - Some cell types grow better than others - Not suitable for studies of matrix invasion and cell–cell interactions - Not useful for high-throughput drug screening - Long-term stability and survival are poorly known , [ , , ] .

Techniques: Comparison, Cell Culture, Gene Expression, In Vivo, Expressing, Drug discovery